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Comparing Genotyping Technologies for Efficiency and Cost-effectiveness
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PINEMAP Research Summary, October 2012, Authors: Ross Whetten, Konstantin Krutovsky, and Jason Holliday.
Several methods for genotyping using high-throughput DNA sequencing have been described using model organisms or annual crops. Application of these techniques to conifers, such as loblolly pine, is not trivial, because the pine genome is several times larger and contains more repetitive DNA than most of the species in which these techniques were developed. As part of the PINEMAP research objectives on genetic analysis of pine populations, we have compared several techniques to evaluate the relative strengths, weaknesses, costs, and benefits of each. No single technique is optimal for every purpose, so the objectives of individual experiments are important in determining which method is best suited to achieve the desired results.
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